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Image Search Results
Journal: Advanced Science
Article Title: Modeling the Effects of Protracted Cosmic Radiation in a Human Organ‐on‐Chip Platform
doi: 10.1002/advs.202401415
Figure Lengend Snippet: Functional and structural changes to eLivs after neutron exposures. A) Schematic of the platform with eLiv tissue being characterized. B) Histological staining of eLivs using canonical markers for hepatocytes (CYP450, CK18), fibroblasts (vimentin), and matrix deposition (COL1A1). C) Albumin and D) urea secretion in eLiv culture supernatant, normalized to the untreated controls. E) Cytokine secretion by eLivs 24 h, 1 week, and 2 weeks post initial radiation exposure, normalized to the untreated controls. Significance was noted by two‐way ANOVAs with multiple comparisons.
Article Snippet: [ ] Sections were washed and permeabilized with 0.25% Triton‐X, blocked in 10% serum, and stained with tissue‐specific primary antibodies. eBM tissues were stained with CD45 (Mouse, AB1430). eLiv tissues were stained with Vimentin (Abcam, ab24525), CK18 (ReVMaB, 31‐1162‐00), anti‐cytochrome P450 CYP3A4 (Sigma, AB1254),
Techniques: Functional Assay, Staining